Being
another lab day, we were subject to wearing laboratory attire: long pants and
closed-toe shoes in the blazing hot weather, but as soon as I entered the
air-conditioned Bio-Med building, all of it became obsolete and I focused on
the day ahead of me. As I mentioned yesterday, today in the lab we worked with
restriction enzymes and gel electrophoresis. Restriction enzymes recognize and
cut specific areas of DNA. Basically, gel electrophoresis uses an electric
current to separate mixtures of the DNA, which shows up as different “bands” on
the gel. This process is used in a wide range of applications, from finding
damaged genes and genetic diseases to tracing paternity lines to examining
tissue samples found at crime sites.
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The lab bench |
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My gel soaking up the rays |
At the end
of today’s session, we used UV light to highlight and take pictures of the DNA
in the gels. Then we compared the results we got to those in the book, which
held pictures of the ideal results of the lab, as well as various different
scenarios of what could’ve gone wrong. This really made us go back and reflect
about the process itself, what each component in the lab was meant for, and
what to keep in mind the next time. Today’s lab however, is only in
anticipation for tomorrow, when we will use the concepts we’ve gained in the last
few days to explore a more complex one: DNA methylation.
Instead of
going to the V-Dubb for lunch, Taylor, Kelly, and I decided to try the
high-praised Korean BBQ truck that we’ve passed by so many times, and it turned
out to be delicious. After finishing, we settled down in the second floor of
the bookstore and strapped ourselves in for the next four hours in order to get
homework done and study. Overall, it was productive and worked out well, and the
time flew by--I even managed to get a short nap in before it was time to go
for dinner. I think that I probably wouldn’t have been able to work as
efficiently on my own.
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Korean Beef Galbi and Kimchi Dumplings |
Our class
took the initiative today by scheduling a study group at 7:30 PM. Everyone was
freaking out about tomorrow’s quiz, and our goal was to make sure we were on
the same page in preparing for it. When we told
Jody about our plans beforehand, she seemed surprised but at the same time
pleased. After we found an abandoned meeting room in
one of the upper floors of the Rockefeller Library, we proceeded to set up a student-run seminar,
complete with our own PowerPoint presentation to review. It was very open, cooperative, and
stimulating discussion in which we were able to put all of our concerns out
there and compile notes that we thought were critical from our past few days. At one point
we were even on online chat with our TA Colby, whom we bombarded with questions.Through this teamwork, I think we were able to bond pretty well. There’s a
feeling you get when around students that, have the same goals and drive to excel. It pushes and
motivates you even more, and that’s
exactly how I feel about my class.
I topped off
the evening by heading down to Salomon Hall with my floormate, Aaron and his
friends to watch the NBA finals game on the big screen (we have no TV in any of our dorms). Although I don’t follow basketball much, the
charged and energetic atmosphere coming from crowds of people made for an exciting and entertaining evening.
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Salomon Hall--the OKC/Heat game! |
I look forward to what the next day holds, despite the forecast for a thunderstorm amid 90 degree weather!
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