The first
Friday spent on campus felt just like any other day this week. Today we had our
third lab in five days. The purpose of today’s lesson was to test the effects
of DNA methylation. Methylation occurs when specific methylase adds a methyl
group to its respective restriction recognition site, ‘protecting’ the DNA by
preventing the restriction enzyme from binding and cutting it. To test this
property, we again used gel electrophoresis. Theoretically, if methylase
renders a restriction enzyme unable to cut the DNA, then there should only be
one thick DNA band on the gel rather that several, spread out fragments.
During a
lab, there’s a lot to be aware of manage at the same time. Most of the time we
work on several parts on the lab at once. With so many steps, procedures,
reagents, and hazardous chemicals to handle, we really must be organized
enough to see everything the whole way through. Everyone is well aware of the
fact that one mistake could result in having to start over major sections of
the project, or worse. In the end, my gel produced incorrect results,
leaving me to ponder which section of the 3-hour long process could have possibly gone wrong.
At 1 PM I
had a floor meeting with our RA, so I left everyone else on the green and
hurried back to my dorm. During the meeting we discussed and came up with
activities we wanted to do as a floor. Starting next Friday, we have planned to
be held in one of the undergrad houses. Because I haven’t been staying in my dormitory
too much due to the murderous weather, I haven’t interacted extensively with my
floor mates yet and I look forward to having the chance to get to know them
better.
My new favorite place to study |
Although we
were predicted to have thunderstorms during the day, we saw no sign of it until
early evening. As I went to meet David, who had just woken up from his nap, the
wind picked up and the skies became more ominous—it was obvious a storm was
brewing. It started to drizzle lightly; the rain felt very nice on my humid and
heat-wracked body, and it was a welcome relief. As we made our way up Thayer, the storm picked up intensity, and
we were bombarded by frightening flashes of lightning and thunder. We were
lucky in that literally just seconds after we made it to the V-Dub, the rain
outside became merciless. You could tell several students had been caught off
guard by the rain, coming in completely drenched. Storms on the East Coast have a much different feel than back home.
From inside the V-Dub--its pouring! |
Tomorrow will mark our first weekend in Brown.
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