The first Friday spent on campus felt just like any other day this week. Today we had our third lab in five days. The purpose of today’s lesson was to test the effects of DNA methylation. Methylation occurs when specific methylase adds a methyl group to its respective restriction recognition site, ‘protecting’ the DNA by preventing the restriction enzyme from binding and cutting it. To test this property, we again used gel electrophoresis. Theoretically, if methylase renders a restriction enzyme unable to cut the DNA, then there should only be one thick DNA band on the gel rather that several, spread out fragments.
During a lab, there’s a lot to be aware of manage at the same time. Most of the time we work on several parts on the lab at once. With so many steps, procedures, reagents, and hazardous chemicals to handle, we really must be organized enough to see everything the whole way through. Everyone is well aware of the fact that one mistake could result in having to start over major sections of the project, or worse. In the end, my gel produced incorrect results, leaving me to ponder which section of the 3-hour long process could have possibly gone wrong.
|My new favorite place to study|
Although we were predicted to have thunderstorms during the day, we saw no sign of it until early evening. As I went to meet David, who had just woken up from his nap, the wind picked up and the skies became more ominous—it was obvious a storm was brewing. It started to drizzle lightly; the rain felt very nice on my humid and heat-wracked body, and it was a welcome relief. As we made our way up Thayer, the storm picked up intensity, and we were bombarded by frightening flashes of lightning and thunder. We were lucky in that literally just seconds after we made it to the V-Dub, the rain outside became merciless. You could tell several students had been caught off guard by the rain, coming in completely drenched. Storms on the East Coast have a much different feel than back home.
|From inside the V-Dub--its pouring!|
Tomorrow will mark our first weekend in Brown.